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High Resolution DNA Imaging by Atomic Force Microscopy
Dmitry Klinov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian
Academy of Sciences, Moscow, Russia
Unique resolution was demonstrated in tapping mode
and point contact atomic force microscopy of DNA molecules using
new diamond-like carbon probes with a ~ 1nm extremity. The probes
were prepared by a plasma-assisted growth of carbon spikes at the
apex of conventional Si probes. The probes were grown at the apex
of Si probes, which are usually applied in atomic force microscopy.
TEM micrographs of one of the probes (Figure a) show differently
oriented carbon spikes grown at the Si tip. The most advanced spike
with the ~ 1 nm extremity can be used for AFM imaging. A tip apex
of etched Si probes used in tapping mode is typically in the 10-20
nm range. The resolution in tapping mode images is higher than the
apex size that is most likely related to the fact that tip-sample
contact area depends not only on the tip size but also on imaging
force and mechanical properties of the sample and the tip. Ambient
imaging of the DNA molecules with the diamond-like carbon and Si
probes showed that fine structural resolution is achieved only by
using the sharp diamond-like carbon probes (Figure b). The images
obtained with this probe reproduce the helical pitches and Z-like
kinks on the DNA molecules.
Conductivity of native DNAs of course will depend on many factors, such as
the contacts to the electrodes [1], the procedure of deposition [2]. Different
types of kinks and bendings that is not resolved by using conventional Si probes
can also decrease conductivity of DNAs. Images of DNA molecules obtained with
these probes (Figure b) exhibit high-resolution.
(a) TEM micrographs of the new diamond-like carbon
probes at different magnifications, (b) AFM image of DNA molecules
with the new diamond-like carbon probe on the mica.
1. Kasumov A., Kociak M., Gueron S., Reulet B., Volkov
V., Klinov D., and Bouchiat H., Science, 291, 280-282 (2001).
2. Kasumov A., Klinov D, Roche P.-E., Gueron S., and Bouchiat H., Applied physics
letters, 84 (6), 1007-1009 (2004).
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